Abstract:In order to solve the acetochlor residue,an acetochlor-degrading strain, named AC-1, was isolated from acetochlor-contaminated soil of acetochlor factory by the conventional enrichment culture technique. Based on the morphological characteristics, physiological and biochemical characteristics, and phylogenetic analysis of 16S rRNA gene sequence, the strain AC-1 was identified as Rhodococcus sp. Strain AC-1 could completely degradate 0.2 mM acetochlor in 48 h, but couldn’t mineralized acetochlor. Based on LC-MS analysis, the metabolite of acetochlor degraded by strain AC-1 was identified as 2-chloro-N-(2-ethylphenyl-6-methyl)acetamide (CMEPA). The optimum temperature and pH for acetochlor degradation by strain AC-1 were 30℃and 7.5, respectively. The acetochlor degradation activity of strain AC-1 were strongly inhibited by 0.1 mM Cu2+ and Hg2+, but Fe2+ could increase the degradation ability slightly. Increasing initial inoculum could promote the degradation. Strain AC-1 showed good properties for degrading alachlor and butachlor, but have slight degradation ability to metolachlor. Strain AC-1 could significantly enhance the degradation of acetochlor in soil. This study provided theoretical and technical support about degradation of acetochlor.