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作 者 ：陈剑杰, 曹谨玲, 宋杰, 李潇

期 刊 ：核农学报 2016年 30卷 5期 页码：1030-1035

关键词：氟化钠；鲤鱼；肾；抗氧化酶；Na+-K+-ATPase蛋白表达；

Keywords:sodium fluoride, carp, kidney, antioxidant enzymes, the expression of Na+-K+-ATPase protein,

摘 要 ：为探究氟对鲤鱼的毒性机制,本试验检测了不同浓度(0、40、80、120 mg·L^-1,分别标记为CK、T1、T2、T3)下,水相氟暴露对鲤鱼肾组织抗氧化酶SOD、CAT、LPO含量及Na⁺-K⁺-ATPase活性的影响,并通过免疫印迹法和免疫荧光组织化学法检测了水相氟暴露对其肾组织Na⁺-K⁺-ATPase蛋白表达的影响。结果表明,在不同氟浓度下暴露30 d后,T2、T3的SOD、CAT、Na⁺-K⁺-ATPase活性均显著低于CK,LPO含量呈升高趋势且显著高于CK;Na⁺-K⁺-ATPase蛋白表达分析表明,T2、T3的Na⁺-K⁺-ATPase蛋白的表达显著低于CK。相关性分析表明,氟暴露浓度与SOD、CAT、Na⁺-K⁺-ATPase活性呈负相关(Pearson系数分别为-0.586,-0.707,-0.818,P <0.01),与LPO含量呈正相关(Pearson系数为0.762,P <0.01), 与Na⁺-K⁺-ATPase蛋白的表达呈负相关(免疫印迹法Pearson系数为-0.769,P<0.01,免疫荧光法Pearson系数为-0.848,P<0.01)。结果表明,水体中氟可在一定程度上对鲤鱼肾抗氧化酶和Na⁺-K⁺-ATPase水平产生抑制作用。本研究为更深入研究水氟的毒性效应提供了理论依据

Abstract:In order to study the toxic mechanism of fluoride on carp (Cyprinus carpio), the paper examined the activities of SOD, CAT, Na+-K+-ATPase, the content of LPO and the expression of Na+-K+-ATPase protein using Western blotting and Immunofluorescent techniques in kidney of carp exposed to different concentrations of fluoride (0, 40, 80, 120 mg·L^-1, mark with CK, T1, T2, and T3, respectively) for 30 d.The results indicated that after exposure for 30 d, the activities of SOD, CAT, Na+-K+-ATPase in kidney of carp exposed to 80, 120 mg·L^-1 of fluoride were significantly lower than those in control fish, while the LPO content showed an increasing trend and the LPO content was significantly elevated in kidney when exposed to 80, 120 mg·L^-1 of fluoride compared with the control group. The analysis of Na+-K+-ATPase protein expression suggested that the expressions of Na+-K+-ATPase protein were remarkably decreased in kidney of carp after exposure to 80, 120 mg·L^-1 of fluoride compared with the control fish. Correlation analysis indicated that the fluoride concentration was negative correlated with the activities of SOD、CAT、Na⁺-K⁺-ATPase (Pearson coefficients were-0.586,-0.707 and-0.818 respctively, P<0.01) and the expression of Na+-K+-ATPase protein(Western blotting pearson coefficient was-0.769, the pearson coefficient of immunofluorescent techniques was-0.848, P<0.01), while was positively correlated with LPO content(Pearson coefficient was 0.762, P<0.01). All above indicated that exposure to fluoride in water could inhibit the antioxidant enzymes and Na⁺-K⁺-ATPase level in kidney of carp to some extent, which will provide theoretical basis for the further study of toxic effects of fluoride in water.

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