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作 者 ：叶晓青, 佘建明, 贾新平, 梁丽建, 刘晓青

期 刊 ：核农学报 2016年 30卷 5期 页码：835-840

关键词：蕨类植物；绿色球状体；成苗；60Co-gamma；射线辐照；SSR分子标记；

Keywords:pteridophyte, greenglobularbodies(GGB), shooting, 60Co-&gamma, raysirradiation, SSRmarker,

摘 要 ：

为开创蕨类植物新的育种方法,以鸟巢蕨、扇蕨和凤尾蕨等3种蕨类植物的绿色球状体为材料,在附加0.2mg·L^-1 IBA的改良1/2MS培养基上,研究不同剂量的⁶⁰Co-γ射线辐照对绿色球状体成苗的影响。结果显示,⁶⁰Co-γ射线辐照剂量在60Gy时,辐照处理的鸟巢蕨绿色球状体成苗率从0Gy(CK)的98%降至33%;剂量在80Gy时,辐照处理的扇蕨和凤尾蕨绿色球状体成苗率分别从CK的100%降至38%和26%,因此60~80 Gy的⁶⁰Co-γ射线为辐照处理蕨类植物绿色球状体的适宜剂量。SSR分子标记测定结果表明,鸟巢蕨绿色球状体经Gy 60和 80 Gy的⁶⁰Co-γ射线辐照诱变获得的植株与对照植株在DNA水平上存在显著差异,其中SSR-23和SSR-76可作为筛选鸟巢蕨突变体的适宜引物。本研究建立的蕨类植物绿色球状体⁶⁰Co-γ射线辐照诱变技术,为在离体器官水平上开展蕨类植物辐照诱变育种提供了条件。

Abstract:The green globular bodies (GGB) of Asplenium nidus, Neocheiropteris palmatopedata(Baker)Christ and Pteris cretica were used for ⁶⁰Co-γ rays irradiation. In the 1/2 MS modified medium supplemented with 0.2 mg·L^-1 IBA, GGB were irradiated at the dose rate 1 Gy·min^-1 of ⁶⁰Co γ-rays. The results showed that the shooting rate was from 98% to 25% when GGB irradiated with 0 and 60 Gy in Asplenium nidus. The shooting rate was from 100% to 38% and 26% when GGB irradiated with 0 or 80 Gy in Neocheiropteris palmatopedata(Baker)Christ and Pteris cretica respectively. The suitable dosage of ⁶⁰Co-γ rays were 60 to 80 Gy for shooting from GGB in pteridophyte. The result of SSR markers test demonstrated that the specific SSR markers were associated with the mutants, among which, SSR-23 and SSR-76 were suitable for identification for screening mutants in Asplenium nidus. In summary, the mutation technique of ⁶⁰Co-γ rays irradiation has been established in GGB of pteridophyte.

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