Abstract:Lophopyrum elongatum is an important wild relatives of common wheat (Triticum aestivum L.), which bears a variety of genes responsible for biotic and abiotic stress resistance. For the purpose of inducing expression of stress response and tolerance genes, Lophopyrum elongatum seedlings were treated with 100 mmol L-1, 200 mmol L-11, 300 mmol L-11, and 400 mmol L-11 of NaCl and 20% (w/v), 30% (w/v), 50% (w/v) of PEG-16000, the full-1length cDNAs were synthesized by using a modified SMART(switching mechanism at 5‘end of RNA transcript)method. And a mixed full-1length entry cDNA library of Lophopyrum elongatum was constructed with BP reaction in Gateway Technology, the titer is 4.0×106 cfu mL-1, the capacity is 2.0×107 cfu, with an average of 0.75 kb insert fragments and 98.9% of recombination rate. This entry cDNA library was subsequently shuttled into the plant transgenic binary vector of pMDC83 to obtain the destination library by LR reaction. The original titer of the destination library was 6×106 cfu mL-11, the capacity is 3.0×107 cfu, the average length of insert fragment was 0.45 kb and the recombination rate was 100%. The transgenic T0 generation seeds of maize were obtained by pollinating the pollens which were germinated and infected by Agrobacterium carrying the destination cDNA library. The transgenic rate was 3%. Meanwhile, the destination library was transformed into BY-12 cells through co-1incubation of Agrobacterium and BY-12 cell. 69 salt tolerant cell lines were obtained by screening with homomycin resistance coupled with 50 or 100 mmol L-11 NaCl. This study established a rapid and high throughput method for screening and identification of the salt resistance genes at both plant level and cell level.