Abstract:A pot experiment was conducted by using four levels of N and S applied in factorial combinations of 5, 10, 20, and 40mmol·L-1 and 1, 2, 4, and 8mmol·L-1 in vermiculite culture to investigate the effects of nitrogen and sulfur interaction on garlic seedlings growth and antioxidant enzymes activities. The results indicated that plant height, stem diameter, root activity, biomass and distribution, antioxidant enzymes (POD, CAT, SOD) activities in leaf and root increased firstly and then decreased, with the increasing concentration of nitrogen and sulfur interaction. The demand for nitrogen, N1 level was optimal; for sulfur, S2 was the most suitable at garlic seedling stage. When the nitrogen content in more than 20mmol·L-1 or sulfur concentration more than 4 mmol·L-1, plant growth was retarded. Nitrogen and sulfur had no significant effect on distribution of biomass and antioxidant enzymes activities of garlic seedling leaf. However, the influence of nitrogen and sulfur interaction on roots and antioxidant activities in leaves of garlic seedlings reached significant or extremely significant level. The results showed that the nutrient solution containing 5mmol·L-1 N and 2mmol·L-1 S was optimal for garlic seedlings, and two elements with obvious interaction effect. The study provides practical theory basis for fertilization on the garlic seedling stage and ‘plant factory‘ pattern.