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作 者 ：李美茹, 曾纪晴, 王以柔, 刘鸿先, 林植芳

期 刊 ：热带亚热带植物学报 2001年 9卷 3期 页码：256~261

关键词：木本植物；光强；活性氧；脂氧合酶；黄嘌呤氧化酶；

Keywords:Woody plants, Light intensity, Active oxygen, Lipoxygenase, Xanthine oxidase,

摘 要 ：将亚热带自然林的乔木荷树、黧蒴和灌木九节、罗伞幼苗栽种于3种不同光强下分析叶片中的LOX、XOD、MAO活性和分离叶绿体中和活性氧O₂^-.、OH·和H₂O₂的产生速率差别的结果表明:全自然光下生长的叶片中LOX和XOD活性最高,降低光强则2个酶的活性下降。叶绿体中的3种活性氧的产生随光强变化的趋势与LOX及XOD活性变化相似。这反映高的LOX和XOD活性是高光强下叶片中出现较高活性氧水平的原因之一。供试灌木的LOX和XOD活性低于乔木,但其活性及活性氧形成速率对光强变化的敏感性高于乔木。

Abstract:The seedlings of two tree species (Schima superba and Castanopsis fissa )and two shrub species (Psychotrla rubra and A rdisia quinquegona) from a subtropical natural forest were grown in pots under 100％，36％ and 16％ natural light． The difference in activities of lipoxygenase (LOX)， xanthine oxidase (XOD) and monoamine oxidase (MAO) in leaves and the production rates of three active oxygen species (O₂^-., OH·, H₂O₂) in chloroplasts were c0mpared．Leaves grown under 100％ sun light showed highest activities of LOX and XOD．The changing trends ofO₂^-., OH· and H₂O₂ in chloroplasts responding to light intensity were significant and similar to that of LOX and XOD activities．High activities of LOX and XOD might be one of the causes of high active oxygen level in leaves that were adapted to high light intensity．The tested understory shrub plants showed lower LOX and XOD activities than the canopy tree species， but the response of enzyme activity and active oxygen production to the change of light intensity was more sensitive than tree species.

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