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作 者 ：成雄鹰;高明尉;梁竹青;

期 刊 ：核农学报 1987年 1卷 03期 页码：51-57

关键词：组织培养；诱变；水稻；

摘 要 ：本试验以两个仙稻品种为材料,研究成熟种子经γ射线、EMS和SA(叠氮化钠)处理后的离体培养反应。实验结果表明,γ射线和EMS、SA明显降低种子出愈率。除γ射线外,其它处理对新诱导成的愈伤组织平均重量无降低作用,而且这三种诱变因子均具提高愈伤组织生长速度的效应。在广陆矮四号中,诱变处理可提高绿点型愈伤组织的比例,γ射线和EMS处理还可提高愈伤组织再生能力。根据本实验的结果,初步认为籼稻成熟种子在培养前的适宜处理剂量(浓度)为:γ射线5—10kR,EMS<0.2M,SA 2—4mM。

Abstract:Dry seeds of two rice cultivars were treated with gamma-rays (0-40 kR),EMS (0-0.4M)and SA (0-4mM) and then inoculated in N6 medium containing 0.5mg/l 2,4-D,4mg/l NAA and 2mg/l 6-BA to induce callus.Growth and differentiation‘ of the callus treated rice seeds were observed in subsequent subcultures.Decreased callus induction percentages were observed in treatments with gamma-rays,EMS and SA,but the mu-tagenic treatments did not reduce the mean weight of primary calli except that the seeds were irradiated with gamma-rays.Moreover,up to 3-fold increase in callus growth rate was shown after treatments of the three mu-tagenic agents.Higher proportion of calli with green spots or small shoots was also seen in one cultivar treated with the three mutagens.Signif icant increase in plant regeneration capacity was found in 60-day-old calli derived from gamma-rays or EMS-treated rice seeds in one cultivar.The results suggested that the following dose or concentration can be used for explant treatment in indica rice before culture,gamma-rays.5-10kR,EMS.<0.2M and SA:2-4mM.

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