Abstract:Esterase isozyme band E3S and E3F is specified by two codominant alle--les of Est3 in rice respectively.Mabanuo,a variety of rice,shows stable 12A esterase isozyme band when it was not subjected to radiation.The M2 of Mabanuo,when it was subjected to radiation of 26 krad and 44 krad,respectively,shows three esterase isozyme types,namely 12A ( the type of slow band),13A (the type of rapid band ) and 12 A/13A ( the type of com-plemental band ).The result shows that the mutation frequency of Est3S to Est 3F allele under 26 krad and 44 krad irradiation is 3.5% and 5.06% respectively.