Abstract:The effects of sulfur and pH(5.0-8.0) on photohydrogen production was investigated in a unicellular green alga, Chlorella pyrenoidosa. Under continuous illumination of 165 μmol m-2s-1 conditions, in the TAP culture medium, changes of chlorophyll a content、Fv/Fm and ΦPSII indicated that growth of C. pyrenoidosa were very well at initialcultivation pH ranged from 6.0 to 7.0 and can form a temporary anoxic conditions in favor of H2 production. Maximum H2 production was obtained at initial cultivation pH 7.0 by C. pyrenoidosa. The maximum rate of H2 produced by C. pyrenoidosa was 0.10 ml mg-1 chl h-1 and the maximum total yield of H2 produced was 1.39 ml. In TAP-S culture medium, changes of chlorophyll a content、Fv/Fm and ΦPSII indicated that growth of C. pyrenoidosa were inhibited and can form a permanent anoxic conditions in favor of H2 production. The total yield of H2 production by C. pyrenoidosa in TAP-S culture was more than that in TAP culture. The Fv/Fm value of C. pyrenoidosa in pH 5.5 TAP-S culture was higher than the others culture. It indicated the residual PSII was more than the others. Under continuous illumination the electron of production H2 requirement mostly comes from PSII, so the maximum rate and total yield of H2 produced by C. pyrenoidosa appeared in pH 5.5 TAP-S cultures. The maximum rate of H2 produced by C. pyrenoidosa was 0.58 ml mg-1 chl h-1 and the total yield of H2 produced was 10.98 ml. The H2 production of C. pyrenoidosa was notable effected by sulfur and pH. In this test the best conditions for H2 production of C. pyrenoidosa was in pH 5.5 TAP-S cultures.