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作 者 ：易厚富， 刘兵， 范云， 王宏斌， 刘良式， 王金发

期 刊 ：热带亚热带植物学报 2001年 9卷 3期 页码：185~189

关键词：水稻；广亲和品种；cosmid文库；

Keywords:Rice, Wide compatible variety, Cosmidlibrary,

摘 要 ：用改进的方法成功构建了一个高质量的水稻广亲和品种(Cpslo17)的核基因组cosmid文库。文库以SuperCos1为载体,克隆总数约为12万以上,克隆平均插入片段约40kb,空载率约为5%,文库容量覆盖水稻单倍基因组约9倍以上。用RFLP标记23D12R作为探针,从文库中分离到一个位于水稻广亲和基因座S5附近的克隆R2I19。

Abstract:A genomic cosmid library of the wide-compatibility-variety rice (Oryza sativa L.Cpslo17) had been generated using vector SuperCos1. The library containing more than 1.0×10⁵ recombinants with an average insert size of approximately 40 kb represented more than 9 times haploid genomic equivalents, but 5% clones did not carry any insert. Using RFLP marker 23D12R linked tightly to S₅ as probeto screenlibrary, a new clone R2I19 was isolated and determined.

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