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作 者 ：张明永，梁承邺，黄毓文，黎垣庆

期 刊 ：热带亚热带植物学报 1994年 2卷 2期 页码：71~76

关键词：雄性不育水稻；自由基代谢；育性转换；幼穗分化；

Keywords:Male sterile rice, Free radical metabolism, Fertility transferring, Panicledifferentiation,

摘 要 ：光（温）敏核雄性不育水稻（Ｐｈｏｔｏｐｅｒｉｏｄ／ｔｅｍｐｅｒａｔｕｒｅ－ｓｅｎｓｉｔｉｖｅｇｅｎｉｃｍａｌｅ－ｓｔｅｒｉｌｅｒｉｃｅ，简称Ｐ／ＴＧＭＲ）８９０２５和培矮６４ｓ在秋季从不育阶段经半不育阶段向可育阶段转变进程中，对其花粉母细胞形成期（Ⅴ）、花粉母细胞减数分裂期（Ⅵ）的幼穗和成熟花药（Ⅷ）中过氧化物酶（Ｐｅｒｏｘｉｄｓｅ，简称ＰＯＤ）、超氧物歧化酶（Ｓｕｐｅｒｏｘｉｄｅｄｉｓｍｕｔａｓｅ，简称ＳＯＤ）．和过氧化氢酶（Ｃａｔａｌａｓｅ，简称ＣＡＴ）活性测定表明，幼穗和花药相同发育时期的酶活性随正常花粉的比率升高而增加，但以成熟花药中增加最多．并伴有丙二醛（Ｍａｌｏｎａｌｄｅｈｙｄｅ，简称ＭＤＡ）含量减少．表明自由基代谢的变化可能与光（温）敏核雄性不育水稻花粉败育有关，且自由基代谢变化在幼穗发育中期即已开始．

Abstract:The activity of peroxidase, catalase, superoxide dismutase and the content ofmalonaldehyde in panicles and anthers of P/TGMR 8902s and Petal 64s were analysed at three different developmental stages (pollen mother cell formation, V; pollen mother cell meiosis, VI;and mature anther, Vlll)during transferring from sterility to fertility. The results showed that the activity of the three enzymes in fertile pened were higher than that in sterile period. In addition, the content of malonaldehyde decreased with the increasins rate of normal pollens. It is concluded that the changes of activated oxygen metabolism in P8nicles and anthers of P/TGMR may be related to pollen abortion in P/TGMR.

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