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作 者 ：胡峰1,2；施琼1；黄烈健1*

期 刊 ：植物研究 2015年 35卷 2期 页码：179-184

关键词：厚荚相思；腋芽；组织培养；玻璃化；

Keywords:Acacia crassicarpa, axillary, tissue culture, verification,

摘 要 ：以16年生厚荚相思优树新生带腋芽茎段为外植体进行组织培养和快繁体系建立的研究，结果表明：含腋芽茎段经75%酒精和0.1%升汞分别处理30S和12min后，接入MS+蔗糖2%上进行初代培养，存活率为67.33%，芽诱导率为80.56%；低浓度的活性炭可有效缓解组培苗玻璃化现象，最佳增殖培养基为改良MS+6-BA 0.5 mg·L^-1+NAA 0.25 mg·L^-1+Ac 0.1 g·L^-1+蔗糖3%，35 d增殖倍数可达3.72；最佳生根培养基为改良MS+IBA 0.5 mg·L^-1+蔗糖2%，生根率为98.83%；将生根苗移栽至以黄心土为基质的营养杯中，存活率为90.00%。

Abstract:We studied the tissue culture system of Acacia crassicarpa by using the stemsegment with buds collected from 16-years plant as explants. The buds could be induced successfully on 2% of MS+sucrose with 67.33% of the survival rate and 80.56% of shooting rate after sterilized with 75% of alcoho1 and 0.1% of mercuric chloride for 30 s and 12 min, respectively. Low concentration of activated carbon effectively alleviated the verification, the best medium for multiplication was Modified-MS+6-BA 0.5 mg·L^-1+NAA 0.25 mg·L^-1+Ac 0.1 g·L^-1+sucrose 3% and the multiplication number was 3.72 after 35 days. The best medium for rooting was Modified-MS+IBA 0.5 mg·L^-1+2% of sucrose, and the rooting rate was 98.83%. The survival rate reached 90.00% after transplanting seedlings to the nutrition cup with yellow soil.

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