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作 者 ：刘志雄1,2；李凤兰2

期 刊 ：植物研究 2015年 35卷 4期 页码：535-539

关键词：樱；花发育；重瓣花；SEEDSTICK；

Keywords:Prunuslannesiana, flowerdevelopment, doubleflower, SEEDSTICK,

摘 要 ：用同源克隆方法，从日本晚樱（Prunus lannesiana）花芽中克隆出了PrseSTK基因的cDNA全长，GenBank登录号为GU332504。其包括1个共669 bp的完整开放阅读框，编码222个氨基酸和1个终止密码子。同源序列比对和分子系统进化分析表明，PrseSTK是拟南芥的STK同源基因，其编码蛋白的C末端拥有2个高度保守模体：AG motif Ⅰ和Ⅱ，属D类MADS-box转录因子。其在花器官中表达的组织特异性分析表明，在单瓣‘大岛樱’中，PrseSTK主要在雄蕊和雌蕊中表达；但在重瓣品种‘普贤像’中，其在萼片、雄蕊和叶化雌蕊中均有表达。其在2个品种4轮花器官中的表达呈现明显的差异，并与拟南芥STK基因表达的组织特异性也有一定的差别；其在花萼中的异位表达可能与重瓣品种萼筒异位子房的发育调控相关。

Abstract:To find out genes involved in the floral organogenesis of Prunus lannesiana, we cloned cDNAs of one MADS-box genes, PrseSTK with GenBank accession No.GU332504 by using homologous cloning. The full length of PrseSTK contained an open reading frame(ORF) of 669 bp and coding for a polypeptide of 222 amino acid residues. By sequence comparison and phylogenetic analysis, PrseSTK was highly homologous to protein STK in Arabidopsis, and belonged to class D. However, by semiquantitative RT-PCR, PrseSTK was highly expressed in stamen and gynoecium of single-flowered P.lannesiana cultivar ‘Makino’, and in sepal, stamen and leaf-like gynoecium of doubleflowered cultivar ‘Albo-rosea’, which was significantly different from the expression pattern of STK in Arabidopsis. The different expression of PrseSTK in double flower showed ectopic ovules formation would result from PrseSTK expression in the sepal.

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