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作 者 ：王珂；刁健；杨瑞；胡彦波*；孙广玉

期 刊 ：植物研究 2015年 35卷 6期 页码：843-847

关键词：紫丁香；硝酸盐；光合诱导；气孔开度；暗呼吸；光呼吸；

Keywords:Syringa oblata seedling, nitrate, photosynthesis induction, stomatal opening, dark respiration, photorespiration,

摘 要 ：以哈尔滨市绿化植物紫丁香（Syringa oblata Lindl.）为研究对象，借助便携式光合测定系统和电子显微镜，研究了叶面喷施硝酸盐（50 mmol·L^-1 NaNO₃溶液）对叶片气孔动态（气孔分布、气孔开度）和光合生理过程（光合—呼吸的光诱导、光化学效率、叶绿素含量以及细胞间隙二氧化碳浓度、气孔导度）的影响。结果表明：叶片上、下表皮均有气孔、腺毛分布，喷施处理第5 d时，叶片的气孔开度和导度、光合速率、叶绿素含量等未受到显著影响，但14 d时，气孔关闭、导度下降，光合—呼吸的光诱导过程受到显著影响。

Abstract:With Syringa oblata Lindl. seedling, a greening tree species in Harbin, we investigated the effects of leaf-spraying nitrate(50 mmol·L^-1 NaNO₃) on stomatal behavior and photosynthetic physiology by gas-exchange and electron microscope techniques. We analyzed stomatal distribution in upper and lower epidermis and stomatal opening as well as photosynthesis-dark respiration induction, photochemical efficiency, chlorophyll content, intercellular CO₂ concentration and stomatal conductance. The stomata and glandular hairs are distributed in the upper and lower epidermis, the effects of leaf-spraying depend on treatment time. For five-day treatment, there were no significant differences in the parameters of stomatal behavior and photosynthesis between the treated and controlled leaves. However, 14-day treatment significantly declined stomatal opening and conductance, and inhibited photosynthesis-dark respiration.

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