Abstract:As the most widely distributed Mycorrhizal type, arbuscular mycorrhizal plays a very important role in plant growth and environmental improvement. Many studies confirmed that the arbuscular mycorrhizal can affect the process of plant secondary metabolism, and change the secondary metabolites. We screened three kinds of arbuscular mycorrhizal fungis, and chose three different sources of amur cork seedings as the research object in the inoculation experiment. By combining the different species and different kinds of sources, we explored the impact of the arbuscular mycorrhizal fungi on the growth and alkaloid content of amur cork and its difference among different provenances. The growth and the alkaloid content of amur cork with arbuscular mycorrhizal fungis are better than those of the control group. The mycorrhizal fungi can effectively promote the growth of amur cork and the accumulation of alkaloid content. Overall, it is the Glomus mosseae that affects the content of berberine and jatrorrhizine mostly, and for palmatine it is Glomus intraradices. From the experimental results, there is difference among provenances in the impact on alkaloid with arbuscular mycorrhizal, and the difference may be related to the provenance.