摘 要 :采集东北地区(红花尔基、黑河、漠河)3个地理种群樟子松(Pinus sylvestris var. mongolica)的种子在温室进行育苗和培养,通过连续4年温室持续高温控制培育实验。研究了极端高温(+15℃,+18℃,+21℃)对不同地理种群樟子松幼苗的株高、基径、根冠比、生物量分配及非结构性碳水化合物(NSC)含量的影响,探明樟子松对高温的耐受程度。结果表明:不同地理种群间幼苗的株高、基径、生物量累积存在显著差异,株高、基径、生长速率和总生物量都表现为:黑河(He)(+15℃)>红花尔基(Ho)(+18℃)>漠河(M)(+21℃);根冠比大小依次为:红花尔基(Ho)(+18℃)>黑河(He)(+15℃)>漠河(M)(+21℃)。可溶性糖和淀粉百分含量在同种群不同器官,及不同种群相同器官间均有差异。可溶性糖与淀粉在不同种群间均表现为:黑河(He)(+15℃)>红花尔基(Ho)(+18℃)>漠河(M)(+21℃),可溶性糖与淀粉在不同地理种群的相同器官中均表现为:叶>根>茎。黑河樟子松种群的幼苗对高温胁迫表现出较强的适应能力,具有比红花尔基和漠河樟子松更强的抗高温胁迫的生理基础,意味着樟子松幼苗可适应升高15℃的高温环境,而升高20℃则对其产生严重胁迫,是东北地区适应未来全球气候变暖的理想樟子松种源。
Abstract:Pinus sylvestris var. mongolica seedlings were collected from three diverse geographic populations, Honghuaerji, Heihe and Mohe of Northeast China. As the study subjects, these seedlings were grown up for four years in greenhouse of Northeast Forestry University under the stress of long-term high-temperature cultivation experiments by artificial control. The effects of long-term extreme high-temperature stress (+15℃, +18℃, +21℃) on plant height, base diameter, root-shoot ratio, biomass allocation and non-structure narbohydrates (NSC) of P.sylvestris var. mongolica seedlings were studied, The degree of P.sylvestris var. mongolica tolerance to high temperature was ascertained. Our results showed that significant differences (P<0.05) was found among the different geographic populations of plant height, base diameter, root-shoot ratio, biomass allocation. Among diverse geographic populations, the largest plant height, base diameter and biomass allocation was found in Heihe population, the lowest was in Mohe population. But the largest root-shoot ratio was in Honghuaerji population, the lowest was in Mohe population. There were significant differences among both soluble carbohydrate and starch of different organs in the same population and different populations of the same organ. The largest proportion of soluble carbohydrate and starch content among the three different populations was in Heihe, and the lowest proportion was in Mohe. Among the different organs, the largest proportion of soluble carbohydrate and starch content was in the foliage, and the lowest proportion was in stem. Our study demonstrated that the P.sylvestris var. mongolica seedings from Heihe showed strong ability to adapt long-term high temperature stress, had stronger physiological basis of resistance to high temperature stress compared with the other two populations, it indicated that P.sylvestris var. mongolica seedlings can adapt the temperature rise in 15℃, while the temperature rise in 20℃ could produce severe stres, which was the ideal P.sylvestris var. mongolica provenance in the future of the global climate warming of Northeast China.