Abstract:The preliminary study was carried out on the anther culture of Erigeron breviscapus. The results showed that during callus induction, 60 g·L-1 sucrose was suitable；MS medium was better than B5; the highest induction efficiency 36.03% was achieved on the medium of MS+NAA 1.0 mg·L-1+BA 0.5 mg·L-1+sucrose 60 g·L-1. calli were transferred on medium MS+6-BA 1.0 mg·L-1 to proliferation. After differentiating and taking roots, plantlets were obtained. The chromosome numbers from root-tip cells of regeneration plant was nine. It showed that the regeneration plant was haploid.