Abstract:With the leaf and one-year twig of four evergreen garden tree species as the experimental materials，disposed through low temperature，relative leakage of electrolytes of leafs and twigs，super-oxide dismutase(SOD) activity and peroxidase(POD) activity and malondialdehyde(MDA) content were measured and compared. The results showed that:the relative leakage of electrolytes of leaf and twig increased with decreasing disposed temperature,but the relative leakage of electrolytes of leaf was higher than the twig’s to the same tree species; Mal-ondialdehyde(MDA) contents of Euonymus japonicus Thunb first descended(-20~0℃) then increased but MDA contents of other tree species increased with decreasing disposed temperature; Superoxide dismutase(SOD) activites of tree species first increased then descended but peroxidase(POD) activites descended with decreasing disposed temperature.The cold-resistance ability of the plants were comprehensively judged with all indexes,there were obvious differences among the clones of the ability of cold resistance.The ability of cold resistance on the plants from high to low was Euonymus japonicus Thunb.> Magnolia grandiflora L.> Ligustrum lucidum Ait.> Ligustrum quihoui Carr.