Abstract:The effect of low temperature stress on antioxidant enzyme activity under different conditions were studied. The results showed that the relative membrane permeability and content of MDA were higher than the control. The activitie of SOD under low temperature was higher than the others and the highest level under 5℃/24 h light. The activity of CAT maintained relatively high level under low temperature and different light conditions. It was higher under 5℃12 h light/12 h dark than other groups. The activity of POD was higher under low temperature with light and was lower under dark than the control. The POD、SOD、CAT activity increased under chilling. This might be one of the reason for strong resistance to chilling in Buxus sempervirens.