Abstract:This assay studied the seasonal dynamic of the agronomic traits and the content of alkaloid of the cultivated Catharanthus roseus in a growth season. It showed that the height, biomass and the leaf numbers of C.roseus presented the similar growth tendency, they all had three obviously seasonal growth characteristic, namely the earlier period (May-July) the growth of them was slow, the intermediate stage (July-September) it was fast and the later period (September-October) it was slow. The trend of the total of flower also showed the similar growth characteristic, but in later period (September-October) it was vapidly dropped. The seasonal dynamic of the content of three kinds of alkaloids in the leaves of C.roseus was obvious, and the tendency was consistent, namely increased first, reduced and increased again, moreover, the content of three kinds of alkaloid appeared two obvious crest values in the late July and October. There were strongly positive correlation of the vinblastine content with catharanthine and vindoline content in the leaves of C.roseus（L.）G. Don, moreover, the correlation between the content of vinblastine and vindoline was marked (p<0.05), and there was remarkably positive correlation between the content of catharanthine and vindoline (p<0.05). Therefore, the best harvest season for artificial planted C.roseus is in the late of October, and we can induce the increase of vinblastine analogues by changing the environment at factors.