Abstract:78 species and varieties in 30 genera and 18 families of liverworts are reported based on specimens from Gansu Province. Among them, 35 species, 11 genera and 3 families (Radulaceae, Wiesnerellaceae, Pallavicinaceae) are new to Gansu Province. Floristic analysis shows that North temperate elements take up the majority, approximately 43.24%, followed by East Asian elements, accounting for 22.97%. The floristics of liverworts in Gansu Province obviously shows temperate characters.