---

作 者 ：杨逢建;庞海河;张学科;孙佳音;祖元刚*

期 刊 ：植物研究 2007年 27卷 5期 页码：556-558

关键词：光胁迫；南方红豆杉；叶绿体色素；紫杉醇；

Keywords:light stress, Taxus chinensis var. mairei, chloroplast pigment, taxol,

摘 要 ：研究了在光胁迫下南方红豆杉叶片中叶绿体色素含量和紫杉醇含量的动态变化。结果表明，与对照相比，叶绿素a、叶绿素b和总叶绿素的含量以及叶绿素a/叶绿素b比值在光胁迫下均减少，随胁迫时间的延长，叶绿素a、叶绿素b和总叶绿素的含量逐渐上升，叶绿素a与叶绿素b的比值先上升后下降；类胡萝卜素含量在前两周与对照相比降低，2周后高于对照；类胡萝卜素与叶绿素的比值高于对照，随胁迫时间的延长，其趋势先降低后上升。光胁迫处理后，南方红豆杉叶片中紫杉醇含量与对照相比，在第1周时略有下降，而后随胁迫时间的延长，紫杉醇含量在第2周开始迅速大量积累，而在处理3周时达到较高值，已增加到1.5倍，这对提高人工种植的南方红豆杉中紫杉醇含量具有非常重要的意义。

Abstract:The changes of the content of chloroplast pigment and taxol in the leaves of Taxus chinensis var. mairei were studied with light stress.The results comparing with the comparison showed that the contents of chlorophyll-a, chlorophyll-b and chlorophyll and the ratio of chlorophyll-a to chlorophyll-b were lower. However, the contents of chlorophyll-a, chlorophyll-b and chlorophyll tended to gradually rise as the stress prolonged, but the ratio of chlorophyll-a to chlorophyll-b first rose and then decreased. The content of carotenoid was lower than the comparison in the first two weeks and then surpassed the comparison. The ratio of carotenoid to chlorophyll was higher than the comparison and tended to first decreased and then rose as the stress prolonged. The contents of taxol was slightly lower than the comparison in the first week and then begun to gradually accumulate in the leaves of Taxus chinensis var. mairei at the second week, the content of taxol had increased to 1.5 times of the comparison at the third week, which is very significant to improve the content of taxol in the growed Taxus chinensis var. mairei.

---