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作 者 ：周小梅;徐谨;李君剑*

期 刊 ：植物研究 2009年 29卷 3期 页码：325-328

关键词：黑麦草；种子；预处理；愈伤诱导；植株再生；

Keywords:Lolium perenne, seeds, pre-treatments, callus, plant regeneration,

摘 要 ：对不同预处理条件下多年生黑麦草种子萌发、愈伤组织诱导以及植株再生进行了研究。结果表明，35℃恒温干燥处理3 h、浸泡16 h后，室温晾48 h的干湿交替及强度为1 000 GS磁场处理30 min均能使种子的发芽率得到显著提高。最适诱导培养基为MS+2,4-D 6 mg·L^-1,有机添加物对于愈伤组织的生长影响并不显著，但可以改善其状态；较为适宜的分化培养基为MS+2 mg·L^-1 6-BA或KT；生根培养基为附加0.5 mg·L^-1 NAA的MS培养基。

Abstract:We studied the effects of pre-treatments on seed germination, callus inducement and the plant regeneration of Lolium perenne. The results showed that pre-treatments including seeds drying 35℃ for 3 h, immersion for 16 hours then drying for 48 h and irradiation under 1 000 Gs magnetic field promoted the germination ratios significantly. MS medium was the optimal one in the three media tested. The 6 mg·L^-1 2,4-D was suitable for inducing callus formation from seeds. The medium for callus differentiation into plantlets contained 2 mg·L^-1 cytokinin (6-BA or KT). The basic MS medium adding 0.5 mg·L^-1 NAA was best for regenerated plantlets rooting.

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