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作 者 ：崔 杰;徐德昌;李滨胜;杨 谦;孙璟晗

期 刊 ：植物研究 2006年 27卷 5期 页码：583-588

关键词：甜菜；叶绿体基因组；atpB基因；克隆与序列分析；

Keywords:sugar beet, chloroplast genome, atpB gene, clone and sequence analysis,

摘 要 ：atpB基因编码ATP合酶β亚基，是光合作用中的重要基因。ATP合酶是生物体内能量代谢的关键酶，参与氧化磷酸化和光合磷酸化反应。利用植物叶绿体基因组在进化过程中高度保守的特点，根据已知植物烟草、水稻和菠菜等的叶绿体基因组全序列，设计并合成了一对引物，以甜菜叶绿体DNA为模板，PCR扩增得到包含atpB 完整基因（GenBank登录号为 DQ067451）在内的一段序列，测序与序列分析表明：该克隆片段全长2 293 bp，其中包括有1 497 bp的编码区序列，推测编码498个氨基酸。同源性比较，该克隆基因与烟草、菠菜、油菜、水稻atpB基因的核苷酸序列同源性分别为90.92%、95.79%、87.71%和86.37%，推测的氨基酸序列同源性分别为94.58%、97.19%、92.17%和91.97%。同时，建立了几种植物的氨基酸序列系统进化树。

Abstract:atpB gene coding ATP synthase beta subunit,it’s a significant gene in photosynthesis. ATP synthase is a key ferment of energetic supersession in organism.It participates in oxidative phosphorylation and photophosphorylation.Based on the highly conservative of plant chloroplast genomesin evolution, the PCR primers were designed and synthesized according to the corresponding sequences in the chloroplast genomes of Nicotiana tobacum, Spinacia and Oryza sativa for amplifying and cloning DNA fragment containing the chloroplast atpB gene (with accessory No.DQ067451 in GenBank respectively) from Beta vulgaris. The sequence analysis results indicated that the length of the determined nucleotide sequence was 2 293 bp,the coding region of atpB gene contained 1 497 bp, which was deduced coding 498 amino acids. Its DNA sequence homologies were 90.92%、95.79%、87.71% and 86.37%, respectively ,with those of Nicotiana tobacum、Spinacia、Brassica napus and Oryza sativa, deduced amino acid sequence homologies were 94.58%、97.19%、92.17% and 91.97%. Amino acid phylogenetic tree was constructed.

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