Abstract:To discuss the ploidy-breeding of multiploid Dioscorea zingiberensis C.H.Wright, the density of upper and lower epidermal cells, cellular figure coefficient, stomata size, stomata density, stomata index and stomata length/width were compared among diploid(2x=20), triploid(3x=30), tetraploid(4x=40) D.zingiberensis. The results indicated that positive correlation was showed between epidermal cells density, stomata area, length and width of stomata, width of guard cells, number of chloroplast in guard cells and ploidy. The significant positive correlation was showed between stomata area, width of guard cells and ploidy, so as one of the methods to identify ploidy of D.zingiberensis, the two can be used.