Abstract:With ISSR method, the paper studied genetic diversity of 60 individuals in two populations of Pinus koraiensis plantation in Benxi CaoHekou forest farm. Through the amplification of ISSR with 14 primers, 90 loci in which 65 were polymorphic were detected and percentage of polymorphic loci was 0.722 2. Nei’s index showed the levels of genetic diversity of Xiquegou population (0.278 9) was higher than Lieshimu population (0.271 2); Shannon’s index got the same result with Nei’s. This study confirmed that P.koraiensis plantation in CaoHekou forest farm save the more genetic diversity.