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作 者 ：冯　璐;栾雨时*;范永强

期 刊 ：植物研究 2007年 27卷 4期 页码：509-512

关键词：银杏外种皮；提取物；抑菌实验；MIC/MBC；

Keywords:ginkgo testas, extracts, inhibiting experiment, MIC/MBC,

摘 要 ：采用菌丝生长抑制法，研究了银杏外种皮3种不同提取物对病原菌（Cylindrocladium colhounii）的抑制作用并测定了这些提取物的MIC/MBC。结果表明，在相同实验天数内，抑菌效果（最小抑菌率）由高到低依次为银杏外种皮乙醇提取物（37.4%）、石油醚提取物（23.7%）和新鲜外种皮汁液（18.4%）。当提取物的浓度提升到一定程度时，三者的抑菌率均可达到100%；三种提取物的MIC/MBC分别为：86.25/86.25,172.5/276,293.25/345 mg·mL^-1。可见银杏外种皮具有明显抑制该病原菌生长的作用，且抑菌成分在乙醇中的溶解度较大。这为进一步研究该抑菌成分乃至生物农药的开发奠定了基础。

Abstract:Three kinds of extracts from ginkgo (Ginkgo biloba L.) testas were examined for the inhibiting effects against plant pathogen Cylindrocladium colhounii using mycelium growth inhibition method. MIC and MBC of three kinds of extracts were also determined. The results showed that, in the same experiment days, ethanolic extracts exhibited the best inhibiting effect and its minimum efficacy is 37.4%, followed by petroleum ether extracts with a minimum efficacy of 23.7%, and the minimum efficacy of fresh extracts is 18.4%. When extracts concentration comes to an appropriate value, three kinds of extracts could achieve an efficacy of 100%. MIC and MBC of three kinds of extracts were 86.25/86.25 172.5/276 and 293.25/345 mg·mL^-1, respectively. The results indicated that the inhibiting components of ginkgo testas dissolved in ethanol more easily than in other two solvents, which provides scientific reference for the application of extracts from ginkgo testas as biological fungicide in agriculture.

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