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作 者 ：曾卫军;原慧;覃建兵*

期 刊 ：植物研究 2012年 32卷 6期 页码：744-749

关键词：小分子热激蛋白；非生物胁迫；抗逆；

Keywords:small heat shock proteins, abiotic stress, stress tolerance,

摘 要 ：小分子热激蛋白是植物受到热胁迫后的主要表达产物之一，与植物细胞耐热有密切关系。该研究发现，拟南芥小分子热激蛋白基因AtsHsp17.6-CⅠ和AtsHsp17.6-CⅡ 除热激之外，重金属离子Ni⁺、Pb²⁺、Cu²⁺、Zn²⁺和Al³⁺均能诱导这2个热激蛋白基因的表达；氧化胁迫和渗透胁迫同样也能诱导它们表达。该研究将由CaMV35S启动子驱动的这2个小分子热激蛋白基因导入拟南芥，RT-PCR分析表明，2个小分子热激蛋白基因在转基因植物中呈现组成型表达。实验结果表明，组成型表达小分子热激蛋白基因AtsHsp17.6-CⅠ的转基因植物表现出对6 μmol·L^-1 Cd²⁺胁迫、0.4% NaCl胁迫的耐受性。研究表明，这2个小分子热激蛋白基因可能参与着多种抗逆途径，推测其能够减轻或抵抗逆境胁迫引起的伤害并对其进行修复。

Abstract:Small heat shock proteins are main products of plants under heat shock stress and have close relationships to the thermotolerance of plant cells. It was showed that two small heat shock protein genes of Arabidopsis thaliana L., AtsHsp17.6-CⅠ and AtsHsp17.6-CⅡ also can be induced by other heavy metal ions, such as Ni⁺, Pb²⁺, Cu²⁺, Zn²⁺, Al³⁺, etc. Osmotic and oxidative stress can also induce their expression. Two kinds of constructions with the two genes under the control of the cauliflower mosaic virus 35S promoter, were induced into the genome of A.thaliana L. respectively. RT-PCR analysis indicated that the constitutive expression of the two genes in transgenic plants. It was showed that AtsHsp17.6-CⅡ gene was over-expressed in A.thaliana, the transgenic plants have acquired stress-tolerance to 6 μmol·L^-1 Cd²⁺ and 0.4% NaCl. Thus, they appear to play major role in maintaining cell function and survival during multiple stress or facilitating recovery from stress.

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