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作 者 ：毛艳萍;苏智先*;邹利娟;吴庆贵

期 刊 ：植物研究 2010年 30卷 1期 页码：106-110

关键词：跳舞草；不定芽增殖；组织培养；快速繁殖；

Keywords:Codariocalyx motorius(Houtt.), multiplication of adventitious buds, tissue culture, rapid propagation,

摘 要 ：以跳舞草无菌苗为实验材料，以MS、1/2MS为基本培养基，研究不同条件对跳舞草快速繁殖的影响，初步建立了跳舞草组培快繁体系，筛选出最佳愈伤组织诱导及不定芽分化培养基为MS+6-BA 2.0 mg·L^-1+NAA 0.1 mg·L^-1+蔗糖30.0 g·L^-1+VC 2.0 mg·L^-1，最佳增殖培养基为MS+6-BA 2.0 mg·L^-1+NAA 0.05 mg·L^-1，最佳生根培养基为1/2MS+NAA 0.2 mg·L^-1+IAA 0.5 mg·L^-1。

Abstract:The aseptic seedings of Codariocalyx motorius(Houtt.) Ohashi were the materials of our study on issue culture and rapid propagation, MS and 1/2MS were the basic mediums. Impact of different conditions on the rapid propagation of C.motorius(Houtt.) was discussed and a tissue culture system of C.motorius(Houtt.) was initially established, the best callus adventitious and bud differentiation medium was MS+6-BA 2.0 mg·L^-1+NAA 0.1 mg·L^-1+sugar 30 g·L^-1+VC 2.0 mg·L^-1, the best medium for the shoot regeneration was MS+6-BA 2.0 mg·L^-1+NAA 0.05 mg·L^-1, the best rooting medium was 1/2MS+NAA 0.2 mg·L^-1+IAA 0.5 mg·L^-1.

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