Abstract:By screening 13 samples being polymorphic with 10 base random primers, we amplified the genomic DNAs of four domestic and three wild cultivars of Lycium barbarum L.. A total of 123 RAPD bands was detected, 68 among which were polymorphic, and the percent of polymorphic bands was 55.28%. Then we calculate the genetic similarity coefficient by Ntsyspc 2.1 and did UPGMA cluster analysis, and found the genetic relationship among the domestic cultivars is close, especially Ningqi No.1 and Ningqi No.2, whose similarity coefficient goes 81.82%, but between the domestic cultivars and the wild cultivars the genetic relationship is far.