Abstract:Because of the differences in organizational structure and chemical composition of various kinds of trees, the nucleic acid extraction method varies as well. High quality DNA is the key factor in the study of forestry molecular biology. Exploring a sort of scientific and appropriate DNA extraction method is very important. In this paper, the extraction method of Populus ussuriensis genomic DNA was optimized. Through the analysis genomic DNA acquired by gel electrophoresis, UV analysis, restriction enzyme digestion and random primer PCR amplification, it is fully proved that it can meet the requirements of molecular biology experiments.