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作 者 ：周露;化文平;王喆之;李翠芹*

期 刊 ：植物研究 2012年 32卷 5期 页码：584-590

关键词：丹参；ACC氧化酶；基因克隆；生物信息学分析；表达模式；

Keywords:Salvia miltiorrhiza, ACC oxidase, gene clone, bioinformatics analysis, expression patterns,

摘 要 ：依据丹参转录组数据库序列信息，采用RT-PCR和染色体步移技术从丹参中首次克隆得到ACC氧化酶基因，命名为SmACO1(GenBank注册号为JQ026111)。该基因gDNA序列长1 347 bp，由3个外显子和2个内含子组成；cDNA全长1 117 bp，包含945 bp的开放阅读框，编码314个氨基酸残基。生物信息学分析显示SmACO1为无信号肽与跨膜结构域，且定位于细胞质的稳定亲水蛋白，含有Fe²⁺依赖的加氧酶结构域。实时荧光定量PCR结果表明，SmACO1基因在丹参不同组织器官中差异表达，花中表达量最高；其表达受到病原菌和茉莉酸甲酯的诱导，表明SmACO1基因可能在植物防御反应中发挥作用。

Abstract:According to the transcriptome database of Salvia miltiorrhiza Bunge, a novel ACO gene was cloned with the method of RTPCR and genome walking for the first time, and named as SmACO1(GenBank accession number: JQ026111). The genomic sequence of SmACO1 is 1 347 bp in length, consisting of 3 exons and 2 introns. The full length of SmACO1 cDNA is 1 117 bp, and has an opening reading frame of 945 bp, which encodes 314 amino acids. Bioinformatics analysis showed that SmACO1 was a stable hydrophilic protein located in the cytoplasm without signal peptide and transmembrane domain, and contains a Fe(Ⅱ)-dependent oxygenase superfamily domain. Quantitative RT-PCR analysis revealed that SmACO1 expressed differently in different organs and the expression level was the highest in flower. Furthermore, this gene could be induced by pathogen and methyl jasmonate, indicating that it might be involved in plant defenses.

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