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期 刊 ：植物保护学报 2009年

关键词：玉米；氯嘧磺隆；敏感性；谷胱甘肽S-转移酶；耐药性；

Keywords:maize, chlorimuron-ethyl, sensitivity, GSTs, tolerant mechanism,

摘 要 ：采用玉米沙培法测定9种玉米品种对氯嘧磺隆的敏感性。结果显示,屯玉88和丰合10对氯嘧磺隆的耐药性较强,IC₅₀值分别为23.98μg/kg和17.83μg/kg;东甜3号的IC₅₀值为0.48μg/kg,对氯嘧磺隆最敏感。氯嘧磺隆对活体ALS的抑制作用远低于离体试验结果,离体条件下不同来源的ALS对氯嘧磺隆的敏感性差异不明显;活体条件下耐药性品种屯玉88和中等耐药品种本育9的ALS活力分别是离体条件下的2.50和2.19倍,而敏感品种东甜3号、东农248和东农888的ALS活力分别是离体条件下的1.02~1.45倍。不同玉米品种ALS和GSTs活性存在差异,耐药性品种ALS和GSTs的活性大于敏感品种。此外,氯嘧磺隆可以诱导GSTs活性增加。

Abstract:This paper determined the drug resistance of 9 maize varieties to chlorimuron-ethyl with sand culture experiment. The results showed that IC₅₀ value for Tunyu 88 and Fenghe 10 was 23.98μg/kg and 17.83μg/kg respectively, the tolerance was strong. Dongtian 3 was sensitive to chlorimuron-ethyl. The IC₅₀ was 0.48μg/kg. The inhibition of chlorimuron-ethyl to ALS in vivo was much lower than in vitro. In vitro, there was no significant difference in the drug resistance of different sources ALS to chlorimuron-ethyl. In vivo, tolerance varieties ALS activity Tunyu 88 and Benyu 9 respectively was 2.5 and 2.19 times than those in vitro, but sensitive varieties Dongtian 3 and Dongnong 248 and Dongnong 888 was 1.02-1.45 times than those in vitro, there was significant difference in maize varieties ALS and GSTs activities, the activities of tolerant varieties ALS and GSTs higher than sensitive varieties. The chlorimuron-ethyl induced GSTs activities increased.

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