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期 刊 ：植物保护学报 2010年

关键词：植物内生细菌；促生；防病；

Keywords:endophytic bacterium, promoting growth, disease control,

摘 要 ：为了获得应用性强的植物内生细菌接菌剂,选用卡拉胶、聚乙烯醇-海藻酸钠(PVA-SA)、草炭和米糠4种载体分别配制了内生细菌菌株XG32、DLJ1和DP24的12种固体菌剂,分别采用利福平标记法和盆栽试验研究了菌剂中内生细菌在辣椒体内的定殖能力和菌剂的促生防病效果。结果表明:卡拉胶和草炭比较适合用作菌剂载体,经卡拉胶包埋和草炭吸附的菌体数量可达10⁸ CFU/g,保存12个月后活菌数均在10⁶ CFU/g以上;制成菌剂后不影响内生细菌在植物体内定殖和促生作用;菌剂的促生试验中处理组与对照组相比,株高、根长、鲜重和干重均差异显著,其中干重增加率为109.03%～140.63%;制成的固体菌剂对辣椒疫霉病的防治效果显著,为75.0%～84.2%。

Abstract:In order to obtain feasible application endophytic bacterium agents, twelve kinds of agents were made respectively by putting the endophytic bacterium strain XG32, DLJ1 and DP24 into carrageenan, polyvinyl alcohol-sodium alginate (PVA-SA), peat and rice chaff. Colonizing dynamics of the bacterium marked with rifampicin were investigated in pepper seedlings. Meanwhile, the growth-promotion and control efficacies also were tested via potted plantlets. The results indicated that carrageenan and peat were more suitable to make entophytic bacterium agents than the other two carriers, the number of bacterium absorbed in carrageenan and peat reached 10⁸ CFU/g, then declined to 10⁶ CFU/g after 12 months. The colonization capacity of entophytic bacterium in plant remained unchanged when the bacterium were made into agents. The plant height, root length, fresh height and dry weight of the plant treated with carrageenan and peat agents were promoted significantly when compared to the control treatment, especially the dry weight increased from 109.03% to 140.63%. The efficacies of the carrageenan and peat agents against Phytophthora capsici were 75.0%－84.2%.

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