Abstract:To enrich Oryza sativa resistance resources against Rice stripe virus (RSV) and accelerate resistant cultivar breeding, 69 indica and japonica varieties from southern China and eastern China were detected with two assays in Zhejiang and Jiangsu: artificial infestation by Laodelphax striatellus which isinsect host of RSV and natural induction of the RSV disease symptoms in the field. Among 69 lines used in this study, 11 cultivars displayed preferable resistance to RSV, as the positive control Zhendao88 did. Genetic analysis showed that the resistance trait was dominant. Interestingly, 3 cultivars possessed the same resistant locus with Zhendao88, while 8 ones did not, demonstrating that the resistance resources were diverse. The offspring of these materials with good resistance and high yield were good parents for breeding of RSV resistant varieties in the distribution area of late O.sativa ssp. japonica in southeastern China.