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期 刊 ：植物保护学报 2009年

关键词：加工番茄；腐霉菌；根腐病；rDNA-ITS区序列分析；鉴定；

Keywords:processing tomato, Pythium, root rot disease, rDNA internal transcribed spacer sequence, identification,

摘 要 ：为了明确新疆加工番茄根腐病的病原种类,从成苗期到果实成熟期对各主栽区的病原物进行分离,并进行形态学鉴定和ITS序列分析。结果表明,从162个根腐病样中获得120个分离物,其中腐霉菌93株,占总分离物的77.5%。腐霉菌分离物鉴定为3 个种,瓜果腐霉Pythium aphanidermatum (Edson) Fitzp,56株,占腐霉分离物的60.22%;简囊腐霉P.monospermum Pringsheim,6株,占6.45%;棘腐霉P.acanthicum Drechsler,2株,占2.15%;另有29个分离物因未诱发出雄器和藏卵器而无法鉴定到种。

Abstract:In order to investigate the pathogens for instructing agricultural production, the pathogens which caused root rot of processing tomato were isolated from diseased processing tomato from seeding stage to fruit ripening stage in the different main production areas. Pythium isolates were identified according to the morphological features of culture and the ITS sequence analyzed of rDNA of representative strains. The results showed that 162 samples of root rot diseased tomato plant were collected and 120 isolates were obtained. Ninety-three of these, 77.5% of the total isolates, belong to Pythium which was the main pathogen for root rot of processing tomato in Xinjiang. These isolates were identified to class into three different species, Pythium aphanidermatum (Edson) Fitzp, P.monospermum Pringsheim and P.acanthicum Drechsler. P.aphanidermatum is 56 strains, accounted for 60.22%, P.monospermum is 6 strains, accounted for 6.45% and P.acanthicum is 2 strains, accounted for 2.15% in the total Pythium isolates. Other 29 strains of Pythium isolates could not be identified due to no oogonium and sporangia induced.

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