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期 刊 ：植物保护学报 2010年

关键词：棉蚜；吡虫啉；哒螨灵；交互抗性；生物学特性；

Keywords:Aphis gossypii, imidacloprid, pyridaben, cross-resistance, biological characteristics,

摘 要 ：为明确哒螨灵对抗吡虫啉棉蚜Aphis gossypii种群的防治效果,采用浸渍法、微量点滴法、生化测定法和系统观察法,研究了哒螨灵对抗吡虫啉棉蚜种群(RF₂₇)、敏感棉蚜种群(SS)和夏津种群(XJ)的负交互抗性及对其生物学特性的影响。结果表明:哒螨灵对RF₂₇、XJ和SS的LD₅₀分别为1.065×10^-5、6.616×10^-5和4.862×10^-5μg/头;抗吡虫啉棉蚜种群与哒螨灵、吡蚜酮具有明显的负交互抗性,与啶虫脒、烯啶虫胺等具有交互抗性;CarE、GST和AChE比活力的提高是引起棉蚜对吡虫啉产生抗性的主要生化原因;哒螨灵对棉蚜的AChE和GST均有显著抑制作用;哒螨灵处理的抗吡虫啉棉蚜种群表现出明显的生殖缺陷,相对适合度较小,为0.67。

Abstract:In order to clarify the control effects of pyridaben on imidacloprid-resistant population of Aphis gossypii, we used the wingless adult aphids of the imidacloprid-resistant population (RF₂₇), the Xiajin population (XJ) and the susceptible population (SS) to assay negative cross-resistance and biological characteristics of pyridaben to imidacloprid-resistant population of Aphis gossypii by using the dipping method, microspot method, biochemical method and systematic observation method, respectively. The results showed that the LD₅₀ of pyridaben to RF₂₇, XJ and SS was 1.065×10^-5μg/aphid, 6.616×10^-5μg/aphid and 4.862×10^-5μg/aphid, respectively. RF₂₇ exhibited obviously negative cross-resistance to pyridaben and pymetrozine, and the RF₂₇ exhibited cross-resistance to other pesticides (acetamiprid, nitenpyram, etc). The enhancement of activities of CarE, GST and AChE would be the main metabolic mechanism of the occurrence of resistance. Pyridaben had obvious inhibition on the AChE and GST activities of Aphis gossypii. The RF₂₇ treated by pyridaben showed developmental disadvantages, the relative fitness was 0.67.

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