Abstract:Bacterial dry stalk rot caused by Pantoea agglomerans, is a new disease in maize seed production field. For understanding the transmission of P.agglomerans in maize seed, the detection of seeds of inbred line Jinyu 9856, male parent line PS056 and female parent OSL190 were conducted using three methods including normal method, Sherlock Microbial Identification System and molecular species-specific detection method. P.agglomerans was detected in and isolated from seeds of PS056 and Jinyu 9856, but not in OSL190. Two isolates were pathogenic and causing dry stalk rot in plants of PS056. P.agglomerans, inoculated onto seeds of PS056 by soaking, injecting and nature infection, can cause typical symptoms of dry stalk rot, and the infection percentage was 100%, 100% and 80%, using three inoculation methods respectively, and there were not symptoms by seed treatment with high temperature (50 ℃) for four days. The result of systematic detection showed that P.agglomerans, inoculated on seeds, can move up to maize plant and it can reach to leaves and seeds through stem along vascular system. All results mean that P.agglomerans successfully cycled from seed to new seed.