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期 刊 ：植物保护学报 2010年

关键词：双重PCR；水稻细菌性谷枯病菌；检测；

Keywords:duplex PCR, Burkholderia glumae, detection,

摘 要 ：根据水稻细菌性谷枯病ITS和gyrB基因,设计两对特异性PCR检测引物,建立了水稻细菌性谷枯病菌的双重PCR检测方法。用该方法对水稻细菌性谷枯病菌和其它植物源性细菌进行双重PCR扩增及灵敏度测试,并对采自不同地区的水稻样本进行水稻细菌性谷枯病菌的检测。结果显示,双重PCR方法能特异性地检测出8株水稻细菌性谷枯病菌,可从含水稻细菌性谷枯病菌浓度为10²cfu/mL的菌液中检测出该病菌;采用该方法对我国不同地区的水稻材料进行检测,并未发现水稻细菌性谷枯病菌。

Abstract:A duplex PCR method was developed for detection of Burkholderia glumae with two pairs of specific primers, which were designed based on ITS (16S-23S rRNA interspacer region) gene and gyrB (β-subunit polyeptide of DNA gyrase) gene. Using duplex PCR method, only B.glumae could be detected,the sensitivity of the method is 10²cfu/mL bacterial cells. There was none B.glumae found in the rice material comes from different areas of China with the douplx PCR method.

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