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期 刊 ：植物保护学报 2009年

关键词：斜纹夜蛾；茚虫威；抗性机制；酯酶；钠离子通道；

Keywords:Spodoptera litura, indoxacarb, resistance mechanisms, esterase, sodium channel,

摘 要 ：以斜纹夜蛾对茚虫威相对敏感种群和抗性倍数为15.63倍的抗性选育种群为材料,通过解毒酶活性测定与钠离子通道基因片段的克隆、测序,研究了斜纹夜蛾对茚虫威的抗性机制。结果表明,与相对敏感种群相比,抗性种群酯酶活性提高了2.27倍,但抗性和敏感种群谷胱甘肽S-转移酶和多功能氧化酶O-脱甲基活性无显著差异;3龄幼虫酯酶活性随着抗性指数的上升而逐渐提高;从相对敏感和抗性斜纹夜蛾种群3龄幼虫基因组DNA中扩增出位于钠离子通道IIS5-IIS6的341bp DNA片段;与相对敏感种群相比,茚虫威抗性种群钠离子通道基因没有发生突变。

Abstract:The resistance mechanisms of indoxacarb-selected population of common cutworm, Spodoptera litura (Fabricius), with resistance ratio of 15.63, were investigated by enzyme assays of esterases (ESTs), glutathione S-transferases (GSTs)and microsomal multifunctional oxidase(MFO)and cloning and sequencing of sodium channel gene. The results showed that the activity of ESTs increased by 2.27-fold compared with the susceptible population, while the activities of MFO and GSTs had no significant difference. Positive correlation was observed between ESTs activity in 3^rd larvae of every selected generation and resistance ratio. DNA fragments of 341bp in length in the IIS5-IIS6 region of sodium channel para-orthologous gene were amplified and sequenced in both resistant and susceptible population, no sodium channel mutation was found in the resistant population compared with the susceptible population.

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