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期 刊 ：植物保护学报 2012年

关键词：交替呼吸途径；间接防御；盐胁迫；过氧化氢；细胞死亡；

Keywords:alternative pathway, indirect defense, salt stress, hydrogen peroxide, cell death,

摘 要 ：为探索在遭受盐胁迫时二斑叶螨Tetranychus urticae Koch侵染叶片的交替呼吸途径的生理学功能,在实验室条件下研究了NaCl以及水杨基氧肟酸对二斑叶螨侵染叶片的间接防御反应,以及对过氧化氢含量和细胞死亡水平的影响。200 mmol/L NaCl胁迫没有明显影响二斑叶螨侵染叶片对智利小植绥螨Phytoseiulus persimilis的吸引作用,也没有提高二斑叶螨侵染叶片中过氧化氢含量和细胞死亡的水平,但显著提高了侵染叶片中交替呼吸途径的水平。200 mmol/L NaCl和1 mmol/L水杨基氧肟酸的复合处理则显著降低了二斑叶螨侵染叶片对捕食螨的吸引作用,并提高了过氧化氢含量和细胞死亡的水平。研究表明,当二斑叶螨侵染叶片遭受盐胁迫时,交替呼吸途径不仅有助于维持植物的间接防御反应,而且缓解了植物体内的氧化压力和细胞死亡。

Abstract:In order to understand the physiological function of the alternative respiratory pathway of the leaves infested by spider mite, Tetranychus urticae Koch, under salt stress, the effects of NaCl and salicylhydroxamic acid were investigated on the indirect defense response, H₂O₂ content, and the level of cell death of the spider mite infested-leaves under laboratory conditions. The results showed that NaCl at 200 mmol/L did not obviously affected the attractiveness of the spider mite infested-leaves to the predatory mites, Phytoseiulus persimilis, nor increased the H₂O₂ content and the level of cell death of the spider mite infested-leaves. However, NaCl significantly increased the level of the alternative respiratory pathway of the infested leaves. The combined treatment of 200 mmol/L NaCl and 1 mmol/L salicylhydroxamic acid significantly decreased the attractiveness of the spider mite infested-leaves to predatory mites and increased H₂O₂ content and the level of cell death. These results suggested that the alternative respiratory pathway could not only contribute to maintain the indirect defense response but also alleviate oxidative stress and cell death of plants in vivo when the spider mite infested-leaves were subjected to salt stress.

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