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,2016,36(5):0964-0970
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犚狅犾犲狅犳犕犻狋狅犮犺狅狀犱狉犻犪犾犃犾狋犲狉狀犪狋犻狏犲犗狓犻犱犪狊犲(犃犗犡)犻狀犘犺狅狋狅狆狉狅狋犲犮狋犻狅狀
犻狀犃狆狆犾犲犇犲狋犪犮犺犲犱犔犲犪犳狌狀犱犲狉犠犪狋犲狉犛狋狉犲狊狊
XUXiuyu1,2,CHENGLailiang1,JINLiqiao2,SUNShan1,LIUMeijun2
(1ShandongInstituteofPomology,Taian,Shandong271000,China;2ColegeofLifeScience,ShandongAgriculturalUniversi
ty,Taian,Shandong271018,China)
犃犫狊狋狉犪犮狋:Thepurposeofthisstudyistoexploretheroleofmitochondrialalternativeoxidase(AOX)in
photoprotectioninappleleavesunderwaterstress.Aftertreatedwithsalicylhydroxamicacid(SHAM)to
inhibittheAOXpathway,westudiedtheeffectsofAOXpathwayonphotoprotectionin犕犪犾狌狊犺狌狆犲犺犲狀狊犻狊
detachedleavesunderwaterstressbysimultaneouslyanalyzingchlorophylafluorescencetransientand
lightabsorbanceat820nm.TheresultsindicatedthatwaterstressinducedtheupregulationofAOXac
tivity.TheinhibitionofAOXpathwaycausedmoreseverephotoinhibition.Underwaterstress,maximum
quantumyieldofprimaryPSⅡphotochemistry(TRo/ABS)andPSⅡtrappedelectronbeingtransferred
fromQAtoQB(ETo/TRo)decreased,averageabsorbedphotonfluxperPSⅡreactioncenter(ABS/RC)
increased,whilethemaximumPSⅠredoxacitity(ΔI/Io)wasnotaffected.AftertreatedwithSHAMto
inhibittheAOXpathway,ABS/RCmarkedlyincreased,TRo/ABS,ETo/TRoaswelasΔI/Iosignificant
lydecreased.ItwasindicatedthatunderwaterstresstheinhibitionofAOXpathwaycausedmoresevere
photoinhibition,especialytothePSⅠ.Generaly,theresultsdemonstratethattheAOXpathwayplayed
animportantroleinthephotoprotectionin犕.犺狌狆犲犺犲狀狊犻狊leavesunderwaterstress,particularlyinthe
photoprotectionofPSⅠ.
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leavesduringdehydrationwithlight
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2 3.36±0.21b 1.11±0.08b 2.25±0.13a 33±2b 67±9b
4 3.78±0.48a 1.45±0.13a 2.34±0.35a 38±3a 62±10b
SHAM
0 2.47±0.25b 0.32±0.05b 2.16±0.21a 13±3d 87±11a
2 2.74±0.33a 0.43±0.03a 2.31±0.3a 16±2d 84±10a
4 2.84±0.32a 0.49±0.05a 2.36±0.27a 17±4d 83±11a
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Aglobaloverviewofdroughtandheatinducedtreemortality
revealsemergingclimatechangerisksforforests[J].犉狅狉犲狊狋
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