Abstract:In order to screen Bacillus spp. with high antagonistic activity towards Sclerotinia sclerotiorum, the causal organism of oilseed rape Sclerotinia disease, the dual culture method was used for screening of high biocontrol activity Bacillus strains. Two strains RJGP16 and YBWC43 were isolated from rhizospheres of grass which collected from Xigaze and Lhasa in Tibet. According to the biochemical tests, physiological tests, 16S rDNA sequences analysis and BOX-PCR fingerprints analysis, RJGP16 and YBWC43 were identified as B.atrophaeus and B.amyloliquefaciens respectively. Biocontrol efficacy of RJGP16 or YBWC43 on oilseed rape Sclerotinia disease in the detached leaves was 50.24% or 100.00%, respectively. The lipopeptide compounds analysis showed that strain RJGP16 produced surfactin and fengycin, while strain YBWC43 produced bacillomycin D and fengycin, indicating that the biocontrol efficacy of these two strains towards S.sclerotiorum were related to the antifungal lipopeptide compounds produced by strains themselves.