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期 刊 ：植物保护学报 2010年

关键词：麦红吸浆虫；滞育；蛋白质；双向电泳；

Keywords:Sitodiplosis mosellana, diapause, protein, 2-dimensional gel electrophoresis,

摘 要 ：为筛选与麦红吸浆虫滞育相关的蛋白,采用双向电泳及图像分析技术分析了麦红吸浆虫滞育前、后和滞育期的蛋白质图谱及其变化。结果表明,在滞育前幼虫的双向电泳图谱上平均可检测到蛋白质点约300个,其它时期约275个,其中多数分布在分子量8.0～50.0kD、等电点4.0～9.0的区域。在所检测到的蛋白质点中,26个在滞育前特异表达;3个在滞育前缺失,其它阶段表达;2个在滞育后200天特异表达;3个在滞育前和滞育解除后表达,滞育期缺失;34个在所有时期均表达,但各时期的表达量存在显著差异。

Abstract:In order to screen diapause associated protein of wheat blossom midge, Sitodiplosis mosellana (Gehin), two-dimensional gel electrophoresis (2-DE) and image analysis system were used to analyze differential expression of proteins extracted from pre-diapause, diapause and post-diapause larvae of wheat blossom midge. On 2-DE gels, about 300 protein spots were detected in the extracts of pre-diapause larvae and 275 for those in each of other stages, and the majority of these spots were located in the area 8.0-50.0kD and pI 4.0-9.0. Among detected proteins, 26 were unique in pre-diapause, 3 were expressed during diapause and post-diapause but missing in pre-diapause, 2 were unique in diapause 200 days, 3 were expressed during pre-diapause and post-diapause but absent in diapause stages, 34 were expressed in all stages but showed different expression levels among larvae in different stages.

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