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期 刊 ：植物保护学报 2006年

关键词：小西葫芦黄花叶病毒；北京分离物；鉴定；外壳蛋白；

Keywords:ZYMV, Beijing isolate, identification, coat protein,

摘 要 ：在北京东郊自然感病的南瓜Cucurbita moschata上获得一病毒分离物(BJ-1),经生物学、血清学和分子生物学鉴定,确定为小西葫芦黄花叶病毒(Zucchini yellow mosaic virus,ZYMV)。为分析其基因组3‘端特性,以发病叶片中提取的总RNA为模板,对基因组3‘端进行RT-PCR扩增,产物克隆到pMD18-T 载体上进行序列分析,共测定了该病毒分离物包括全部CP基因在内的1 269 bp。该分离物CP基因由837个核苷酸组成,编码279个氨基酸。对包括该分离物在内的30个序列的760 bp(含NIb基因3‘端56 bp和CP基因中的704 bp)片段、NIb蛋白与CP蛋白的切割位点、蚜传必需基序的变异、寄主来源及地域来源进行了分析。结果表明,ZYMV不同分离物的基因分型与上述五个因素无明显关系。

Abstract:A virus was isolated from naturally infected Cucurbita moschata in Beijing. It was identified as Zucchini yellow mosaic virus by biological, serological and molecular biological tests. In order to analyzing the characterization of the 3‘ terminal sequences of the genome, the 3‘ terminal sequences were amplified by RT-PCR form total RNA of infected leaves and cloned into pMD18-T vector. The sequences were 1269 bp and contained the coat protein (CP) gene, which consisted of 837 nucleotides encoding 279 amino acids. The analysis was carried out in 30 isolates on their 760 bp segments (containing the 3‘ terminal 56 bp of NIb gene and 704 bp of CP gene), the incising site of Nib protein and CP, the mutation of the sequence which was related to aphid transmissibility, the source of hosts and terrains. The result showed that the gene types of ZYMV were not obviously related to the five factors.

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