Abstract:The effects of phosphorus deficiency stress on physiological characteristic and chromosome response in wheat was studied by determining protective enzyme activities and MDA content and locating the gene controlling protective enzyme activity and MDA content at different developing stages using wheat substitution lines between Chinese Spring (CS) and Synthetic 6x. The results showed that SOD and POD activities, and MDA content increased under phosphorus deficiency stress. The genes promoting SOD activity might be located on 2A, 3B, 2D and 7D chromosome of Synthetic 6x; and the genes promoting POD activity might be located on 2A, 5A, 6A, 7B and 7D chromosome, while the genes inhibiting MDA content might be located on 5A, 2D, 5D and 7D chromosome of Synthetic 6x.