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作 者 ：李耕, 高辉远, 刘鹏, 杨吉顺, 董树亭, 张吉旺, 王敬锋

期 刊 ：植物营养与肥料学报 2010年 16卷 3期 页码：536-542

关键词：氮素；玉米；灌浆期；光合性能；光系统；

Keywords:nitrogen, maize, grain filling stage, photosynthetic performance, photosystem,

摘 要 ：以玉米自交系齐319（Q319）为材料，采用单株盆栽种植方式，借助叶绿素荧光快速诱导动力学曲线和820 nm光吸收曲线，研究了氮素对玉米灌浆期叶片光合性能的影响。2年研究结果表明，在本试验条件下，氮素对Q319灌浆期叶片叶绿素含量无明显提高作用，但其净光合速率（P_n）和单株干物质积累量、子粒产量显著增加。JIP-test分析表明，氮素显著提高了叶片光系统Ⅱ（PSⅡ）反应中心电子供体侧和受体侧性能； 显著提高了Q319 PSⅡ反应中心电子受体侧之后的电子传递链性能，增强了电子由PSⅡ向PSⅠ的分配，从而显著地提高了PSⅡ与PSⅠ之间的协调性。可以认为，施氮后灌浆期叶片叶绿素含量的变化不是P_n提高的主要原因，而两个光系统性能的改善及二者间协调性的提高增强了光合电子传递链的性能是灌浆期P_n升高与成熟期产量增加的主要原因。

Abstract:A pot experiment was carried out to study the effects of nitrogen fertilization on photosynthetic performances in maize leaves of inbreed line Qi319 (Q319) at grain filling stage using simultaneously analyzing chlorophyll a fluorescence transient and light absorbance at 820 nm. Two years‘ results indicate that N fertilization had no effect on chlorophyll contents in the ear leaf at grain filling stage, but significantly improved the net photosynthetic rate（P_n） and single plant dry matter and grain yield (P<0.05) of Q319. JIP-test analyzing showed that the nitrogen fertilization significantly increased the performances of electron donor and acceptor in photosystem Ⅱ (PSⅡ) reaction center (P<0.05). Furthermore, nitrogen fertilization significantly increased extent of performance on electron transport chain after acceptor in Q319 PSⅡ reaction center was significant, and enhanced the distribution of electron transport from PSⅡ to PSⅠ. Consequently, the coordination between PSⅡ and PSⅠwas significantly improved. We considered that, under nitrogen fertilization treatment, the increasing of P_n and yield was not mainly resulted from the change of chlorophyll content at grain filling stage, but from the enhancement in Q319 photosynthetic performances and improvement of the coordination between PSⅡ and PSⅠand performance of electron transport chain.

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