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作 者 ：张可炜;李坤朋;刘治刚;张举仁

期 刊 ：植物营养与肥料学报 2007年 13卷 5期 页码：795-801

关键词：玉米；细胞工程；耐低磷；酸性磷酸酶；磷利用率；

Keywords:maize, cell engineering, low phosphorus tolerance, acid phosphatase, phosphorus-use efficiency,

摘 要 ：以齐319(对照)和来源于齐319的耐低磷突变体99037和99106为材料,在1μmol/L和1000μmol/L.KH₂PO₄两个磷水平下,通过水培实验研究了磷水平对不同基因型玉米磷吸收、利用和酸性磷酸酶活性的影响。结果表明,低磷条件下三个自交系的根冠比均显著增加,植株磷含量明显降低,生物量显著减小,磷利用效率和酸性磷酸酶活性大大提高。但不同基因型间存在显著差异,自交系99037与齐319相比具有植株磷浓度低、磷素再利用能力强、磷利用效率和净吸收量高等特征,是自交系99037磷高效的可能机理。

Abstract:The effect of phosphorus(P) level on P absorption,utilization and acid phosphatase(APase) activity was studied using hydroponic maize seedlings of original line Qi319(control) and low-P tolerance lines 99037 and 99106,obtained from cell engineering technology,under 1 μmol/L or 1000 μmol/L KH₂PO₄.The results showed that under P-stress environment the root-shoot ratio,P-use efficiency and APase activity of seedlings from three inbred lines increased significantly,and P content and biomass decreased obviously,but there were still significant differences among the three maize genotypes.99037 had lower P concentration and higher P-reuse ability,P-use efficiency and net P-accumulation,compared with Qi319,which may be the primary factors leading to higher low-P tolerance of 99037.

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