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Comparative Study on HPLC Fingerprint of Phyllanthus urinaria

叶下珠药材HPLC指纹图谱的比较研究



全 文 :书!"#$%&
,2016,36(6):1206-1215
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犆狅犿狆犪狉犪狋犻狏犲犛狋狌犱狔狅狀犎犘犔犆犉犻狀犵犲狉狆狉犻狀狋狅犳犘犺狔犾犾犪狀狋犺狌狊狌狉犻狀犪狉犻犪
FANShi1,2,ZUOJiapu1,RAOLiqun2
(1LandscapeDepartment,HunanPolytechnicofEnvironmentandBiology,Hengyang,Hunan421005,China;2ColegeofBio
logicalScienceandTechnology,HunanAgriculturalUniversity,Changsha410128,China)
犃犫狊狋狉犪犮狋:TheHPLCfingerprintof犘犺狔犾犾犪狀狋犺狌狊狌狉犻狀犪狉犻犪fromdifferenthabitats,differentharvesttimes,
differentpartswasdeterminedandcomparedbyRPHPLC.Thecomparativestudywasdonewiththe
HPLCfingerprintofYexiazhuCapsulesalso.TherepresentativeHPLCfingerprintof犘.狌狉犻狀犪狉犻犪wases
tablished.Theresultsshowed:(1)Therewere18mutualpeaks,fourofwhichwereidentifiedbyrefer
encesubstance.Thesimilarityof11samplesfromdifferenthabitatswasintherangeof0.89to0.99.(2)
Thesimilarityof7samplesfromdifferentharvesttimeswasgreaterthan0.94.Thepeakareaofmostmu
tualpeaksincreasedwithgrowthperiod,thehighestwasin5October.Itwassuggestedthebestharvest
timewasonearlyOctoberannualy.(3)Thesimilarityofsamplesfromdifferentpartswasanalyzedande
valuated.Thesimilarityofleafandfruitwashigherintherangeof0.98to0.99,andthesimilarityofroot
andstemwasintherangeof0.86to0.87.Lowercontentofcomponentsinrootandstembroughtabout
thedifferenceinsimilarity.Itwassuggestedonlycolectingtheaerialparts.(4)Thechemicalconstituents
ofYexiazhuCapsuleswerenearlyconsistentwiththatofreferenceherbof犘.狌狉犻狀犪狉犻犪,butthepeakarea
ofsomepeaksvariedverymuchbecauseofprocessingofYexiazhuCapsules.So,thismethodissimple,
accurate,andreproducible,whichcanbeusedforthequalitycontrolandevaluationof犘.狌狉犻狀犪狉犻犪andits
Chineseproprietarymedicines.
犓犲狔狑狅狉犱狊:犘犺狔犾犾犪狀狋犺狌狊狌狉犻狀犪狉犻犪L.;highperformanceliquidchromatography;fingerprint
  nop(犘犺狔犾犾犪狀狋犺狌狊狌狉犻狀犪狉犻犪L.)åaæ4n
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Table1 Sampleinformationtableof犘.狌狉犻狀犪狉犻犪L.
ŸšWX
Samplenumber
Ÿšž
Habitatareaofsamples
1 "YZ Wuchang,Hubei
2 [\‡]Zhongshan,Guangdong
3 ^_`a Hangzhou,Zhejiang

m™qr

‡bqšS$,šc€×

ControlMedicinalHerbs
(ChinaInstituteofPharmaceuticalandBiological
Products)
5 [!(d Nanning,Guangxi
6 e(fa Danzhou,Hainan
7 _!ghJian,Jiangxi
8 i(jÄ Kunming,Yunnan
9 (k2Shaoyang,Hunan
10 [\l_Zhanjiang,Guangdong
11 (maChenzhou,Hunan
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狌狉犻狀犪狉犻犪L.)。
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(S1~ S10):20060202B01、
20060102B08、20051001B06、20050904B04、20050902B20、
20050601B12、20050502B23、20050403B02、20050203B21、
20050102B08。
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Fig.1 HPLCfingerprintofmixtureofreferencesubstancesandrepresentativeHPLCfingerprintof犘.狌狉犻狀犪狉犻犪L.
Q2 11…45678|}~i‚†‡ˆ‰
Table2 Resultsofrelativeretentiontimeofmutualpeakof11samples
˜X
Peaknumber
ŸšWXSamplenumber
1 2 3 4 5 6 7 8 9 10 11
•¥¦
Average
1 0.123 0.124 0.123 0.123 0.124 0.124 0.123 0.123 0.124 0.123 0.123 0.123
2 0.319 0.317 0.316 0.316 0.317 0.317 0.318 0.315 0.317 0.316 0.318 0.317
3 0.447 0.444 0.445 0.445 0.445 0.445 0.445 0.445 0.445 0.446 0.445 0.445
4 0.563 0.561 0.562 0.561 0.562 0.562 0.561 0.561 0.562 0.562 0.561 0.562
5 0.652 0.650 0.651 0.652 0.647 0.654 0.653 0.649 0.649 0.652 0.648 0.651
6 0.824 0.830 0.824 0.824 0.821 0.827 0.824 0.829 0.832 0.823 0.838 0.827
7 0.983 0.981 0.984 0.981 0.981 0.982 0.984 0.980 0.982 0.982 0.981 0.982
8(S) 1.000 1.000 1.000 1.000 1.000 1.000 1.000 1.000 1.000 1.000 1.000 1.000
9 1.151 1.148 1.149 1.148 1.150 1.150 1.148 1.147 1.149 1.153 1.148 1.149
10 1.294 1.298 1.290 1.291 1.292 1.292 1.293 1.293 1.295 1.291 1.295 1.293
11 1.418 1.408 1.415 1.409 1.409 1.411 1.408 1.421 1.413 1.407 1.412 1.412
12 1.432 1.426 1.426 1.427 1.428 1.436 1.427 1.426 1.428 1.422 1.433 1.428
13 1.485 1.473 1.473 1.472 1.476 1.476 1.475 1.473 1.478 1.476 1.472 1.475
14 1.515 1.509 1.509 1.513 1.515 1.505 1.506 1.511 1.514 1.524 1.506 1.512
15 1.582 1.570 1.573 1.573 1.576 1.582 1.578 1.580 1.573 1.581 1.572 1.576
16 1.619 1.628 1.618 1.618 1.622 1.622 1.615 1.625 1.622 1.611 1.624 1.620
17 1.641 1.633 1.647 1.638 1.635 1.638 1.647 1.633 1.643 1.634 1.645 1.639
18 1.681 1.672 1.681 1.680 1.677 1.673 1.679 1.687 1.684 1.683 1.686 1.680
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Table3 Resultsofrelativeareaofmutualpeakof11samples
˜X
Peaknumber
ŸšWXSamplenumber
1 2 3 4 5 6 7 8 9 10 11
•¥¦
Average
1 0.157 0.156 0.170 0.157 0.157 0.157 0.158 0.165 0.157 0.156 0.155 0.159
2 0.263 0.267 0.263 0.263 0.265 0.265 0.263 0.263 0.264 0.264 0.262 0.264
3 0.247 0.247 0.251 0.250 0.244 0.246 0.248 0.244 0.246 0.248 0.245 0.247
4 0.127 0.124 0.125 0.120 0.121 0.122 0.123 0.122 0.124 0.123 0.125 0.123
5 0.102 0.107 0.103 0.109 0.103 0.105 0.107 0.104 0.103 0.106 0.103 0.105
6 1.042 1.036 1.031 1.039 1.046 1.037 1.049 1.035 1.029 1.058 1.057 1.042
7 0.616 0.604 0.617 0.611 0.614 0.603 0.615 0.614 0.621 0.615 0.611 0.613
8(S) 1.000 1.000 1.000 1.000 1.000 1.000 1.000 1.000 1.000 1.000 1.000 1.000
9 5.824 5.716 5.786 5.711 6.212 5.921 5.725 5.824 6.023 5.732 5.882 5.847
10 0.465 0.483 0.461 0.463 0.468 0.471 0.463 0.466 0.465 0.463 0.462 0.466
11 0.365 0.367 0.371 0.375 0.370 0.375 0.367 0.373 0.373 0.373 0.367 0.371
12 0.235 0.234 0.238 0.235 0.234 0.241 0.234 0.235 0.238 0.235 0.232 0.236
13 0.202 0.192 0.205 0.198 0.193 0.196 0.192 0.206 0.195 0.208 0.198 0.199
14 0.119 0.125 0.122 0.120 0.126 0.126 0.125 0.123 0.121 0.125 0.122 0.123
15 0.247 0.236 0.241 0.245 0.241 0.250 0.243 0.242 0.243 0.245 0.243 0.243
16 0.773 0.766 0.763 0.764 0.768 0.772 0.766 0.763 0.765 0.761 0.774 0.767
17 1.639 1.644 1.631 1.626 1.647 1.631 1.634 1.646 1.634 1.652 1.647 1.639
18 0.274 0.267 0.281 0.279 0.275 0.275 0.277 0.273 0.281 0.277 0.279 0.276
Q4 11…45678|}~Š‹Œ~Š‹>
Table4 Mutualpeakarearatiooftotalpeakarea
of11samplesof犘.狌狉犻狀犪狉犻犪L.
ŸšWX
Sample
number
1­®
Total
area(106)
–—˜­®
Mutualpeak
area(106)
#„‚
Percentage/%
1 8.83 8.17 92.5
2 9.03 8.65 95.8
3 10.01 9.27 92.6
4 8.81 8.36 94.9
5 18.57 17.31 93.2
6 9.66 8.74 90.5
7 17.65 16.48 93.4
8 12.89 11.77 91.3
9 8.01 7.42 92.7
10 14.40 13.26 92.1
11 9.03 8.20 90.8
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[1] ðb‡ëqpWWq.ðb‡ëqpW(«r)[M].">:
stuSÂÙv
,1978:263.
[2] 8 9.nopýúþú—hˆ„F„üà HPLCz{|k
GH
[D].bc:(`[a%,2007.
[3] w x,yz{,|}~,*.nopÊ%ˆ„àÐøùúû
üJÞFGH
[J].‡b‡qk,1998,23(6):363364.
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inmice[J].犑狅狌狉狀犪犾狅犳犆犺犻狀犲狊犲犕犲犱犻犮犻狀犪犾犕犪狋犲狉犻犪犾狊,1994,
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$ž#$Ê%%\Mòcò.Ž@
,1995:1115.
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3ÅÆ€
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flavonoidsindifferentgrowthperiodandstorageperiodof
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