---

作 者 ：胡建燃，李 平*，秦路鹏，万田田，刘志芳，方玉洁

期 刊 ：西北植物学报 2016年 8期 页码：1648~1653

关键词：恒山黄芪；宁夏枸杞；复合多糖；抗氧化；羟自由基；超氧阴离子自由基；

Keywords:Hengshan Astragalus, Lycium barbarum, compound polysaccharides, antioxidative activities, hydroxyl radical, superoxide anion radical,

摘 要 ：以恒山黄芪和宁夏枸杞为材料，采用超声波法提取其多糖成分，比较分析恒山黄芪多糖、宁夏枸杞多糖以及二者复合多糖的抗氧化活性，为开发新的天然抗氧化剂提供依据。结果显示：（1）恒山黄芪多糖的总抗氧化能力［T AOC =(0.67±0.12) U/mg］和超氧阴离子自由基清除能力（EC₅₀ = 2.34 mg/mL）均高于宁夏枸杞多糖［T AOC =(0.52±0.07) U/mg，EC₅₀ = 4.28 mg/mL］，而恒山黄芪多糖的羟自由基清除能力（EC₅₀ = 1.36 mg/mL）则低于宁夏枸杞多糖（EC₅₀ = 0.98 mg/mL）。（2）在相同浓度下，二者按照1∶1比例配成的复合多糖总抗氧化能力［T AOC＝(0.79±0.02) U/mg］、羟自由基清除能力（EC₅₀＝0.84 mg/mL）、超氧阴离子自由基清除能力（EC₅₀＝1.37 mg/mL）均高于单种多糖。（3）恒山黄芪多糖、宁夏枸杞多糖以及二者复合多糖的抗氧化能力均与其浓度呈正相关关系。研究表明，恒山黄芪多糖具有较好的抗氧化活性，是具有开发潜力的天然抗氧化物质，且恒山黄芪多糖与宁夏枸杞多糖复合配伍后具有协同效应，抗氧化活性增强。为开发新的天然抗氧化剂提供参考。

Abstract:Polysaccharides were extracted from Hengshan Astragalus and Lycium barbarum by ultrasonic method, and then antioxidative activities of Hengshan Astragalus polysaccharides, L. barbarum polysaccharides and the compound polysaccharides of them were accessed in this study. The results showed that: (1)The total antioxidant capacity ［T AOC =(0.67±0.12) U/mg］ and superoxide anion radical scavenging capacity (EC₅₀ = 2.34 mg/mL) of Hengshan Astragalus polysaccharides were both higher than those of L. barbarum polysaccharides ［T AOC =(0.52±0.07) U/mg, EC₅₀ = 4.28 mg/mL］, while hydroxyl radical scavenging capacity (EC₅₀ = 1.36 mg/mL) was lower than that of L. barbarum polysaccharides (EC₅₀ = 0.98 mg/mL). (2) T AOC, hydroxyl radical scavenging capacity and superoxide anion radical scavenging capacity of the compound polysaccharides were significantly higher than those of any single polysaccharide. (3) Both any single polysaccharide and compound polysaccharides exhibited a dose dependent effect. In conclusion, Hengshan Astragalus polysaccharides show remarkably high antioxidative activities, and are natural antioxidants with development potential. Besides, the compound polysaccharides of polysaccharides from Hengshan Astragalus and L. barbarum (1∶1) enhance the antioxidative effects. Thus, this study may offer reference for further exploration of Hengshan Astragalus polysaccharides and natural antioxidants.

---